Efficient label-free chemiluminescent immunosensor based on dual functionalcupric oxide nanorods asperoxidase mimics

Juan Li,1 Yue Cao,1 Samuel S. Hinman,2 Kristy S. McKeating,3 Yiwen Guan, 1 Xiaoya Hu,1 Quan Cheng,*,2,3 and Zhanjun Yang*,1

1School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, P.R. China

2Environmental Toxicology and 3Department of Chemistry, University of California, Riverside, California, 92521, United States

Abstract:Dual-functionalcupric oxide nanorods (CuONRs) as peroxidase mimicsare proposed for the development of a flow-through,label-free chemiluminescent (CL) immunosensor.Forming the basis of this cost-efficient, label-free immunoassay, CuONRs, synthesized using a simplehydrothermal method,were deposited onto epoxy-activated standard glass slides, followed by immobilization of biotinylated capture antibodies through a streptavidin bridge.The CuONRs possess excellent catalytic activity, along with high stability as a solid support.Antigens could then be introduced to the sensing system, forming large immunocomplexes that prevent CL substrate access to the surface, thereby reducing the CL signal in a concentration dependent fashion. Usingcarcinoembryonic antigen (CEA)as a model analyte,the proposed label-free immunosensor was able to rapidly determineCEAwith awide linear range of 0.1–60 ng mL-1 and a low detection limit of 0.05 ng mL-1.This nanozyme-based immunosensorissimple, sensitive, cost-efficient, andhas the potential to be a very promising platform for fast and efficient biosensing applications.

Biosens. Bioelectron., 2018, 100, 304-311, IF: 7.780

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