Ultrasensitive electrochemical detection of H₂O₂in living cells based on ultrathin MnO₂nanosheets

Yun Shu,¹Jing Xu,¹Jingyuan Chen,¹Qin Xu,¹Xiao Xiao,¹Dangqin Jin,²Huan Pang,*¹Xiaoya Hu*¹

¹College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, P.R.China

²Department of Chemical Engineering, Yangzhou Polytechnic Institute, Yangzhou 225127, P.R.China

^*Corresponding author. Email: huanpangchem@hotmail.com, xyhu@yzu.edu.cn

Abstract

Using a facile and efficient method, two-dimensional (2D) ultra-thin MnO₂nanosheets were successfully prepared. Due to thelarge specific surface area and mesoporous structure, MnO₂nanosheet is a good candidate for use as an enhanced electrochemical sensing material. MnO₂nanosheets were immobilized onto glassy carbon electrodes with Nafion film to construct a H₂O₂electrochemical nonenzymatic sensor. Amperometric study showed MnO₂nanosheets exhibited very high electrocatalytic activity towards H₂O₂reduction. A very low detection limit (5 nM) was reached with a wide linear range (25 nM~2 μM and 10~454 μM) and a high sensitivity of 3261mA•M⁻¹•cm⁻².The fabricated H₂O₂sensor also exhibited excellent selectivity, good reproducibility and long-time stability. Furthermore, the constructed high sensitive sensor was successfully applied to perform real-time monitoring trace concentration of H₂O₂released by SP2/0 cells, indicating that MnO₂nanosheets provide a new platform for developing high performance electrochemical sensors in biological applications.

Sensors and Actuators B: Chemical 252 (2017) 72–78. IF=5.401

文章链接:http://www.sciencedirect.com/science/article/pii/S0925400517309474