Platinum nanoparticle-assembled nanoflake-like tin disulfide for enzyme-based amperometric sensing of glucose

Lei Wang,1 Juan Li,1,* Yunfan Pan,1 Lingfeng Min,2 Yongcai Zhang,1 Xiaoya Hu,1 Zhanjun Yang1,* *

1School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China

2Clinical Medical School of Yangzhou University, Subei People's Hospital of Jiangsu Province, Yangzhou, Jiangsu 225001, PR China

Abstract: We have prepared a platinum nanoparticle-assembled nanoflake-like SnS2 nanocomposite of the type PtNP@SnS2 as a support for immobilization of glucose oxidase (GOx) to obtain an electrochemical glucose biosensor based on direct electron transfer of GOx. Scanning electron microscope, transmission electron microscope, X-ray diffraction, electrochemical impedance spectroscopy, static water contact angle measurements, and cyclic voltammetry were used to characterize the nanocomposite and the glucose biosensor. The nanocomposite has large surface-to-volume ratio, excellent hydrophiliciy, and high electrochemical activity. This promotes the direct electron transfer between GOx and surface of a glassy carbon electrode (GCE). The GOx immobilized on the nanocomposite displays good bioactivity, and the surface coverage of modified electrode is 4.15×10-11 mol·cm-2. Under optimum conditions and at a working voltage of -0.4 V vs. SCE, the glucose biosensor exhibits a linear response in the 0.1to 1.0mM and 1.0 to 12 mM glucose concentration ranges, with a low detection limit of 2.5 μM (at an S/N ratio of 3). The biosensor shows excellent selectivity and was successfully applied to the determination of glucose in human serum samples.

Microchim. Acta, 2017, doi:10.1007/s00604-017-2209-0, IF: 4.831

文章链接:https://link.springer.com/article/10.1007/s00604-017-2209-0

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