MoS₂Nanosheets-Au Nanorods Hybrids for Highly Sensitive Amperometric Detection of H₂O₂in Living Cells

Yun Shu,^aJingyuan Chen,^aQin Xu,^aZhen Wei,^aFengping Liu,^aRui Lu,^bSheng Xu,^bXiaoya Hu*^,a

^aSchool of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China

^bCo-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, School of veterinary medicine, Yangzhou University, Yangzhou 225002, China

Abstract:MoS₂nanosheets-Au nanorods (MoS₂-Au) hybridswere utilized toimmobilize catalase (CAT) to construct a sensitivehydrogen peroxide (H₂O₂) electrochemical biosensor for the reliable determination of H₂O₂released from living cells. The fabricated biosensor was characterized by transmission electron microscopy (TEM), UV–vis spectroscopy, Fourier transform infrared spectroscopy (FT-IR) and Raman spectroscopy, it is observed that MoS₂-Au hybrid provides excellent matrixes for the adsorption of CAT and the entrapped CAT maintains its native structure and bioactivity. The results of direct electrochemical measurements indicate that on the CAT/MoS₂-Au modified electrode, CAT exhibits a surface controlled and a fast electron transfer process towards H₂O₂reduction. The MoS₂-Au hybrid has a large surface area and provides a biocompatible microenvironment for accelerating direct electron transfer between the enzyme and the electrode. Thedetection limit of theconstructed H₂O₂biosensorreaches 1×10^-7M (signal-to-noise=3) with a wide linear range from 5×10^-7M to 2×10^-4M and a high sensitivity of187.4mA•M⁻¹•cm⁻².The fabricated H₂O₂biosensor also exhibits excellent selectivity, good reproducibility and long-time stability. Furthermore, the biosensor was used to perform real-time monitoring of H₂O₂released from SP2/0 cells, indicating the MoS₂-Au hybrid is an attractive material for application in the efficient immobilization of biomolecules and construction of high-performance biosensors.

J Mater Chem B, 2017, 5, 1446-1453.

文章链接:http://pubs.rsc.org/en/content/articlepdf/2017/tb/c6tb02886a