Smart CuS nanoparticles as peroxidase mimetics for the design of novel label-free chemiluminescent immunoassay

Zhanjun Yang*,Yue Cao, Juan Li, Mimi Lu, Zhikang Jiang, Xiaoya Hu*

SchoolofChemistryand Chemical Engineering,YangzhouUniversity

Abstract:A novel label-freechemiluminescent (CL) immunoassaymethod was designed based onsmartCuS nanoparticles(CuSNPs) as peroxidase mimetics.The CuSNPs were synthesized by a facile coprecipitation method, and showed high catalytic activity and stability. This efficient label-free CL immunoassay could be easily achieved through a simple strategy. Firstly, CuSNPs dispersed in chitosan were dropped on the epoxy-activated glass slide to form a solid CL signal interface. Streptavidinwas then used tofunctionalize CuSNPs to capture the biotinylated antibody, further producing a sensing interface. After on-line incubation with antigen molecules, the formed immunocomplex on the sensing interface effectively blocked the diffusion of CL substrate into the signal interface and further inhibited the mimic enzymatic CL reaction, thus leading to the decrease in CL signals. The peroxidase mimetics based label-free CL immunoassay system showed high sensitivity, excellent specificity, acceptable reproducibility and good accuracy. This research provided a new and promising strategy for the development of highly efficient label-free CL immunoassay system.

ACS Appl. Mater. Interfaces 2016, 8, 12031-12038

文章链接: http://pubs.acs.org/doi/pdf/10.1021/acsami.6b02481